EAA – Protein Muscle Growth

Total grams of muscle build by dosage

Values were calculated from measured change from muscle fractional synthetic rate 3h post ingestion, assuming a muscle mass of 30kg. Calculated as described in ref1.

1. Church DC, Ferrando A, Wolfe RR. Stimulation of muscle protein synthesis with low-dose amino acid composition in older individuals. Front. Nutr., 2004 March 08; Sec. Nutrition and Metabolism Volume 11- 2024. https://doi.org/10.3389/fnut.2024.1360312

2. Prior formulation no Arginine and lower leucine, Paddon-Jones D, Sheffield-Moore M, Katsanos C, Zhang X, Wolfe RR. Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein. Exp Gerontol 2006; 41:215-219.

3. Witard OC, Jackman SR, Breen L, Smith K, Selby A, Tipton KD. Myofibrillar muscle protein synthesis rates subsequent to a meal in response to increasing doses of whey protein at rest and after resistance exercise. Am J Clin Nutr, 2014 Jan; 99(1):86-95. doi: 10.3945/ajcn.112.055517. Epub 2013 Nov 20. PMID: 24257722

Our EAA’s outperform other protein synthesis by up to 32x.

Protein muscle growth effect of different foods



Mixed Meal

Ground Beef

Kidney Beans

Mixed Nuts

Peanut Butter

Pork Loin

Beef Sirloin


The tested effects of 3.6 g of our EAA formula on the stimulation of muscle protein synthesis. Older subjects (see Table 1) were studied before and after oral ingestion of the EAA formula.

Subjects were studied during a 5 hr fasted period and for 3 hr after ingestion of the EAA formula. Results indicate that 3.6g of EAA resulted in a significant (p<0.001) 0.056%/hr increase in muscle protein synthesis (Figure 1).

Figure 1. Muscle protein synthetic rates before and after ingestion of 3.6g of EAA. Thus, a small dose of our EAA (3.6g) formula can significantly increase muscle protein anabolism in older subjects.

Footnotes & References

These statements have not been evaluated by the Food and Drug Administration. This product is not intended to diagnose, treat, cure, or prevent any disease.

Iron Remedy MD® product development, procurement procedures, and trial data illustrated are provided through a patented and proprietary formula created by Reganator®. For a complete trial data please contact Iron Remedy MD (click here).

Nutrients doi: 10.3390/nu12123717. Essential Amino Acids and Protein Synthesis: Insights into Maximizing the Muscle and Whole-Body Response to Feeding David D Church 1, Katie R Hirsch 1, Sanghee Park 2 3, Il-Young Kim 2 3, Jess A Gwin 4 5, Stefan M Pasiakos 4, Robert R Wolfe 1, Arny A Ferrando 1.

1. Transmembrane transport and intracellular kinetics of amino acids in human skeletal muscle. Biolo G, RYD Fleming, SP Maggi, RR Wolfe Am. J. Physiol. 268 Endocrinol. Metab. 31:E75-E84, 1995.

2. Insulin Action on Muscle Protein Kinetics and Amino Acid Transport During Recovery After Resistance Exercise. Gianni Biolo, Bradley D. Williams, R.Y. Declan Fleming, and Robert R. Wolfe. Diabetes,Vol 48 May 1999.

3. Exogenous Amino Acids Stimulate Net Muscle Protein Synthesis in the Elderly. Elena Volpi, Arny A. Ferrando, Catherine W. Yeckel, Kevin D. Tipton, and Robert R. Wolfe. J Clin Invest, 101(9):2000-2007, 1998.

4. Nonessential amino acids are not necessary to stimulate net muscle protein synthesis in healthy volunteers. K.D. Tipton, B.E. Gurkin, S. Matin, and R.R. Wolfe. J Nutr Biochem 10:89-95, 1999.

5. Oral amino acids stimulate muscle protein anabolism in the elderly despite higher first-pass splanchnic extraction. Volpi E, B Mittendorfer, SE Wolfe, RR Wolfe. Am J Physiology 277:E513-E520, 1999.

6. Basal Muscle Amino Acid Kinetics and Protein Synthesis in Healthy Young and Older Men Elena Volpi, MD, Melinda Sheffield-Moore, Blake B. Rasmussen, and Robert R. Wolfe. JAMA September 12;286 (10):1206-1212. 2001.

7. Timing of amino acid-carbohydrate ingestion alters anabolic response of muscle to resistance exercise. Tipton KD, BB Rasmussen, SL Miller, SE Wolf, SK Owens-Stovall, BE Petrini, RR Wolfe. Am J Physiol 281:E197-E206, 2001.

8. Essential amino acids and muscle protein recovery from resistance exercise. Borsheim E, KD Tipton, SE Wolf, RR Wolfe. Am J Physiol. 283:E648-E657, Oct 2002.

9. Acute response of net muscle protein balance reflects 24-h balance after exercise and amino acid ingestion. Tipton KD, E Borsheim, SE Wolf, AP Stanford, RR Wolfe. Am J Physiol 284:E76-E79, 2003.

10. Essential amino acids are primarily responsible for the amino acid stimulation of muscle protein anabolism in healthy elderly adults. Elena Volpi, Hisamine Kobayashi, Melinda Sheffield-Moore, Bettina Mittendorfer, and Robert R Wolfe. J Clin Endocrinol Metabo 89:4351-4358, Sep 2004.

11. Independent and Combined Effects of Amino Acids and Glucose after Resistance Exercise. Sharon L Miller, Keven D Tipton, David L. Chinkes, Steven E Wolf and Rober R. Wolfe. Medicine & Science in Sports & Exercise, 2002.

12. Amino acid ingestion improves muscle protein synthesis in the young and elderly. Douglas Paddon-Jones,Melinda Sheffield-Moore, Xiao-Jun Zhang, Elena Volpi1, Steven E. Wolf, Asle Aarsland, Arny A. Ferrando and Robert R. Wolf. Am J Physiol Endocrinol Metab 286: E321-E328, 2004.

13. Essential Amino Acid and Carbohydrate Supplementation Ameliorates Muscle Protein Loss in Humans during 28 Days Bedrest. Paddon-Jones D, M Sheffield-Moore, RJ Urban, AP Sanford, A Aarsland, RR Wolfe. J Clin Endocrinol Metabo 89:4351-4358, Sep 2004.

14. Effect of an amino acid, protein, and carbohydrate mixture on net muscle protein balance after resistance exercise. Borsheim E, Aasland A and Wolfe RR. Int J Sport Nutr Exerc Metab. Jun;14 (3):255-71, 2004.

15. Exogenous amino acids stimulate human muscle anabolism without interfering with the response to mixed meal ingestion. Douglas Paddon-Jones,Melinda Sheffield-Moore Asle Aarsland, Robert R. Wolfe, and Arny A. Ferrando. Am J Physiol 288(4):E761-767, Apr 2005.

16. Aging is associated with diminished accretion of muscle proteins after the ingestion of a small bolus of essential amino acids. Christos S Katsanos, Hisamine Kobayashi, Melinda Sheffield-Moore, Asle Aarsland, and Robert R Wolfe. AM J Clin Nutr 82:1065-73, 2005.

17. Differential stimulation of muscle protein synthesis in elderly humans following isocaloric ingestion of amino acids or whey protein. Douglas Paddon-Jones, Melinda Sheffield-Moore, Christos S. Katsanos, Xiao-Jun Zhang, Robert R. Wolfe. Exp Gerontol 41:215-219, 2006.

18. A high proportion of leucine is required for optimal stimulation of the rate of muscle protein synthesis by essential amino acids in the elderly. Christos S. Katsanos, Hisamine Kobayashi, Melinda Sheffield-Moore, Asle Aarsland, and Robert R. Wolfe. Physiol Endocriol Metab 291:E381-E387, Aug 2006.

19. The deleterious effects of bed rest on human skeletal muscle fibers are exacerbated by hypercortisolemia and ameliorated by dietary supplementation. R. H. Fitts, J. G. Romatowski, J. R. Peters, D. Paddon-Jones, R. R. Wolfe, and A. A. Ferrando. Am J Physio Cell Phsiol 293:C313-C320, 2007.

20. Effect of Amino Acid Supplementation on Muscle Mass, Strength and Physical Function in Elderly Elisabet Børsheima, Quynh-Uyen T. Buib, Sandrine Tissierc, Hisamine Kobayashid, Arny. A. Clin Nutr 27(2):189-195, Apr 2008.

21. Amino acid supplementation decreases plasma and liver triglycerides in elderly. Elisabet Børsheim, Ph.D, Quynh-Uyen T. Bui, M.D., Sandrine Tissier, P.T., Melanie G. Cree, M.D., Ph.D., Ola Rønsen, M.D., Ph.D., Beatrice Morio, Ph.D., Arny A. Ferrando, Ph.D., Hisamine Kobayashi, D.V.M., Bradley R. Newcomer, Ph.D., and Robert R. Wolfe, Ph.D. Nutrition, 25(3):281-288, Mar, 2009.

22. Muscle Protein Synthesis and Balance Responsiveness to Essential Amino Acids Ingestion in the Presence of Elevated Plasma Free Fatty Acid Concentrations Christos S. Katsanos, Asle Aarsland, Melanie G. Cree, and Robert R. Wolfe. J Clin Endocrinol Metab 94(8):2984-90, Aug 2009.

23. EAA supplementation to increase nitrogen intake improves muscle function during bed rest in the elderly. Arny A. Ferrando , Doug Paddon-Jones, Nicholas P. Hays,, Patrick Kortebein Ola Ronsen Rick H. Williams, Ashley McComb, T. Brock Symons, Robert R. Wolfe, William Evans. Clin Nutr 29:18-23, Feb 2010.

24. Reginator demonstrates increase in muscle protein synthesis at low dosage trail of 3.6 grams. Unpublished.

25. Increased nitrogen intake following hip arthroplasty expedites muscle strength recovery. A.A. Ferrando, M.M. Bamman, S.E. Schutzler, H.J. Spencer, A.M. Dawson, R.P. Evans, R.R. Wolfe. Journal of Aging Research & Clinical Practice volume 2, Number 4, 2013.

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